| PMID: 11283337 |
Hihara Y, Kamei A, Kanehisa M, Kaplan A, Ikeuchi M. |
DNA microarray analysis of cyanobacterial gene expression during acclimation to high light. |
Plant Cell. 2001 Apr;13(4):793-806 |
For the exposure to high light (HL) for 15 min and 1 hr, cells grown at 20 μmol photons m–2 sec–1 to a cell density of A730 = 0.1 to 0.2 were transferred to HL (300 μmol photons m–2 sec–1) without dilution. For the exposure to HL for 6 and 15 hr, cells grown in LL were diluted to adjust the cell density to 0.1 (for 6-hr samples) or 0.05 (for 15-hr samples) and transferred to HL. After incubation in HL for 6 or 15 hr, the cell density was A730 ≈ 0.2; thus, self-shading of cells was minimized |
Synechocystis sp PCC 6803 was grown at 32°C in BG11 medium with 20 mM Hepes-NaOH, pH 7.0, under continuous illumination provided by fluorescent lamps. Cells were grown in volumes of 50 mL in test tubes (3 cm in diameter) and bubbled by air containing 1.0% (v/v) CO2. For the low light (LL) samples (growth control conditions), cells were grown at 20 μmol photons m–2 sec–1 for at least 1 day and harvested at a cell density of A730 = 0.6 to 1.0. |
Environmental |
Light Intensity |