Publication or Contributor Authors Title Reference Treatment conditions Control/normal conditions Main source of perturbation Environmental factor tested
PMID: 15805106 Shoumskaya MA, Paithoonrangsarid K, Kanesaki Y, Los DA, Zinchenko VV, Tanticharoen M, Suzuki I, Murata N Identical Hik-Rre systems are involved in perception and transduction of salt signals and hyperosmotic signals but regulate the expression of individual genes to different extents in synechocystis. J Biol Chem. 2005 Jun 3;280(22):21531-8 For exposure of cells to salt stress, a solution of 5.0 m NaCl was added to 50 ml of a suspension of cells that had been grown under standard conditions for 16 h, to give a final concentration of 0.5 m. The duration of incubation under salt stress was 20 min, the same duration and molar concentration (of sorbitol) used for the experiments with hyperosmotic stress Wild-type cells were grown photoautotrophically at 34 °C in 50 ml of BG-11 medium buffered with 20 mm HEPES-NaOH (pH 7.5) under continuous illumination from incandescent lamps at 70 microeinstein m-2s-1, with aeration by air that contained 1% CO2 mutant/Environmetal Osmotic stress