| PMID: 15805106 |
Shoumskaya MA, Paithoonrangsarid K, Kanesaki Y, Los DA, Zinchenko VV, Tanticharoen M, Suzuki I, Murata N |
Identical Hik-Rre systems are involved in perception and transduction of salt signals and hyperosmotic signals but regulate the expression of individual genes to different extents in synechocystis. |
J Biol Chem. 2005 Jun 3;280(22):21531-8 |
For exposure of cells to salt stress, a solution of 5.0 m NaCl was added to 50 ml of a suspension of cells that had been grown under standard conditions for 16 h, to give a final concentration of 0.5 m. The duration of incubation under salt stress was 20 min, the same duration and molar concentration (of sorbitol) used for the experiments with hyperosmotic stress |
Wild-type cells were grown photoautotrophically at 34 °C in 50 ml of BG-11 medium buffered with 20 mm HEPES-NaOH (pH 7.5) under continuous illumination from incandescent lamps at 70 microeinstein m-2s-1, with aeration by air that contained 1% CO2 |
mutant/Environmetal |
Osmotic stress |