| PMID: 18599646 |
Singh AK, Elvitigala T, Bhattacharyya-Pakrasi M, Aurora R, Ghosh B, Pakrasi HB |
Integration of Carbon and Nitrogen Metabolism with Energy Production Is Crucial to Light Acclimation in the Cyanobacterium Synechocystis |
Plant Physiol. 2008 Sep;148(1):467-78 |
For HL treatment, cells grown at LL were transferred in a long test tube (3 cm in diameter) to a cell density of approximately 5 × 107 cells/mL. The tubes containing cells were transferred in a thermostat water bath maintained at 30°C and illuminated with a white-light intensity of 300 μE m−2 s−1 (HL). Cells were air bubbled during HL treatment. Cells from LL- and HL-illuminated cultures were collected after 15 min, 1 h, 2 h, 3 h, 4 h, and 6 h |
Synechocystis cells were grown at 30°C in BG11 medium buffered with 10 mm TES-KOH (pH 8.2) and bubbled with air. Illumination was at 30 μE m−2 s−1 (LL) provided by fluorescent cool-white lights. Cell growth was monitored by measuring OD at 730 nm on a DW2000 (SLM-AMINCO). The growth control condition were the ones defined in treatment as Low light (LL) |
Environmental |
Light Intensity |