| PMID: 18644144 |
Zhang Z, Pendse ND, Phillips KN, Cotner JB, Khodursky A. |
Gene expression patterns of sulfur starvation in Synechocystis sp. PCC 6803. |
BMC Genomics. 2008 Jul 21;9:344 |
Log phase cells (OD 730 nm = 0.6) were harvested by centrifugation (2000×g for 12 min) washed once and then re-suspended in sulfate-free media (MgSO4 replaced by MgCl2 of the same molarity). All S-containing trace metals in BG-11 were replaced by non-S containing metals. Cells were harvested and fixed for microarray analysis by adding 10% (v/v) ice-cold 5% phenol in ethanol stop solution at the following time points: before S-depravation (time 0), 1, 3, 6, 12, 24, 48 and 72 hr after S-depravation |
Synechocystis sp PCC 6803 was grown photoautotrophically in BG-11 medium supplemented with 8 mM NaHCO3 and buffered with 10 mM HEPES (pH 7.4). The cells were grown in 250 ml flasks at 32°C under a light intensity of 25 μmol photons m-2 s-1. Cultures were bubbled with sterile air containing 1% (v/v) CO2 |
Environmental |
Growth stage/Nutrient limitations |