| PMID: 20929957 |
Summerfield TC, Nagarajan S, Sherman LA. |
Gene expression under low-oxygen conditions in the cyanobacterium Synechocystis sp. PCC 6803 demonstrates Hik31-dependent and -independent responses. |
Microbiology. 2011 Feb;157(Pt 2):301-12 |
For microarray experiments, cultures were grown in the bioreactor to a cell density of ∼ 5×10 7 cells ml −1 and then bubbled with 99.9 % nitrogen/0.1 % carbon dioxide to achieve low-oxygen condition. The microarray experiment involved a loop design that compared the wild type and the ΔHik31 strain under aerobic conditions and 1 h following a transition to low oxygen. |
The glucose tolerant strain, Synechocystis sp. strain PCC 6803 was grown at 30±2 °C. Cells were grown in blue green algae medium BG11 with 25 mM HEPES pH 7.5, in an airlift bioreactor (BioFlo 3000, 6 l) or in 750 ml Cytolifts (Kontes). The culture was illuminated by two panels using alternating arrays of orange (640 nm) and blue (430 nm) light-emitting diodes, yielding an intensity of ∼100 μmol photons m −2 s −1 |
mutant/Environmetal |
Low O2 / Normal conditions |