| PMID: 22096149 |
Hackenberg C, Huege J, Engelhardt A, Wittink F, Laue M, Matthijs HC, Kopka J, Bauwe H, Hagemann M. |
Low-carbon acclimation in carboxysome-less and photorespiratory mutants of the cyanobacterium Synechocystis sp. strain PCC 6803. |
Microbiology. 2012 Feb;158(Pt 2):398-413 |
Cultures were bubbled with 5 % CO 2 in air [defined as high C i (HC)] in BG11 medium at pH 8.0, or, alternatively with ambient air [0.035 % CO 2, defined as low C i (LC)] in BG11 medium at pH 7.0. Growth was monitored by measuring OD 750 |
The glucose-tolerant strain Synechocystis sp. PCC 6803 served as the WT. For the physiological and molecular phenotyping, axenic cultures with an OD 750 of approximately 0.8 were grown photoautotrophically in batch cultures at 29 °C under continuous illumination of 100 µmol photons m −2 s −1. |
mutant/Environmetal |
Nutrient limitation |