| PMID: 23275872 |
Hernández-Prieto MA, Schön V, Georg J, Barreira L, Varela J, Hess WR, Futschik ME. |
Iron deprivation in Synechocystis: inference of pathways, non-coding RNAs, and regulatory elements from comprehensive expression profiling. |
G3 (Bethesda). 2012 Dec;2(12):1475-95 |
To induce iron starvation, the chelator desferrioxamine B (DFB, Sigma-Aldrich) was added at a final concentration of 100 µM to bubbled liquid cultures. Samples were taken before induction of iron depletion (control), as well as at 3, 12, 24, 48, and 72 hr after induction of iron depletion. |
Synechocystis wild type strain was grown at 30° in YBG-11, a modified version of BG-11 medium. Light intensity was adjusted to 50 μmol photons m−2·s−1 of white light |
Environmetal |
chemical |