| PMID:19759342 |
Singh AK, Bhattacharyya-Pakrasi M, Elvitigala T, Ghosh B, Aurora R, Pakrasi HB |
A systems-level analysis of the effects of light quality on the metabolism of a cyanobacterium |
Plant Physiol. 2009 Nov;151(3):1596-608. |
White light-grown Synechocystis cells (approximately 1 × 108 cells mL−1) were harvested by centrifugation at 6,000g and resuspended in fresh BG11 medium to a cell density of approximately 5 × 107 cells mL−1. These cells were then transferred in a long test tube (3 cm in diameter) and used for illumination with PSI light (provided by royal blue-emitting diodes) or PSII light (provided by red-emitting diodes). Light intensity was maintained at 10 μE m−2 s−1. Cells were air bubbled during light illumination. Cells were collected after 15 min, 45 min, 1.5 h, 2 h, 3 h, and 6 h following illumination with light. |
Synechocystis species PCC 6803 cells were grown at 30°C in BG11 medium buffered with 10 mm TES-KOH, pH 8.2, and bubbled with air. Illumination was at 30 μE m−2 s−1 provided by fluorescent cool-white light.The growth control condition were the same that treatmente conditions as the fold change of genes was calculated by dividing the normalized intensity obtained from PSII light-illuminated cells by that obtained with PSI light-illuminated cells. |
Environmental |
Light wavelenght |