Publication or Contributor Authors Title Reference Treatment conditions Control/normal conditions Main source of perturbation Environmental factor tested
PMID:26354085 Koskinen S, Hakkila K, Gunnelius L, Kurkela J, Wada H, Tyystjärvi T. In vivo recruitment analysis and a mutant strain without any group 2 σ factor reveal roles of different σ factors in cyanobacteria. Mol Microbiol. 2015 Sep 10 The OD 730 of cell culture was set to 0.1, and the cells were grown in standard growth conditions for 3 days. Cultures (OD730 = 1, 40 ml) were rapidly cooled by pouring them into two frozen centrifuge tubes, and cells were harvested by centrifugation at +4°C. RNA isolation The cells of the glucose-tolerant strain of Synechocystis sp. PCC 6803 were grown in BG-11 medium supplemented with 20 mM HEPES, pH 7.5, at 32°C, under continuous illumina- tion at photosynthetic photon flux density (PPFD) of 40 μmol m−2 s−1 in ambient air. These growth conditions are referred to as standard conditions. Liquid cultures (30 ml) were grown in 100 ml Erlenmayer flasks that were shaken at 90 r.p.m mutant Normal condition(mutant vs wild type)