| PMID:21245330 |
Mitschke J, Georg J, Scholz I, Sharma CM, Dienst D, Bantscheff J, Voss B, Steglich C, Wilde A, Vogel J, Hess WR. |
An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp. PCC6803. |
Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):2124-9. |
Different stress conditions were applied to exponentially growing cultures of Synechocystis 6803 as follows: For high light stress, light intensity was shifted from 50 to 500 μmol of photons•m–2•s–1 for 30min. Another culture was harvested after 1 h incubation in the dark. For CO2-depletion culture was centrifuged and washed one time with BG11 (20mM TES pH 7.0) w/o NaCO2. Culture was harvested after 6h in the CO2 depletion medium without a stream of air. |
Standard growth conditions: Liquid cultures of Synechocystis 6803 were grown at 30°C in BG11 (20mM TES pH 7.6) medium under continuous illumination with white light of 50 μmol of photons•m–2•s–1 and a continuous stream of air. |
Environmental |
Dark/High light/C02-Depletion |