Publication or Contributor Authors Title Reference Treatment conditions Control/normal conditions Main source of perturbation Environmental factor tested
PMID:21245330 Mitschke J, Georg J, Scholz I, Sharma CM, Dienst D, Bantscheff J, Voss B, Steglich C, Wilde A, Vogel J, Hess WR. An experimentally anchored map of transcriptional start sites in the model cyanobacterium Synechocystis sp. PCC6803. Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):2124-9. Different stress conditions were applied to exponentially growing cultures of Synechocystis 6803 as follows: For high light stress, light intensity was shifted from 50 to 500 μmol of photons•m–2•s–1 for 30min. Another culture was harvested after 1 h incubation in the dark. For CO2-depletion culture was centrifuged and washed one time with BG11 (20mM TES pH 7.0) w/o NaCO2. Culture was harvested after 6h in the CO2 depletion medium without a stream of air. Standard growth conditions: Liquid cultures of Synechocystis 6803 were grown at 30°C in BG11 (20mM TES pH 7.6) medium under continuous illumination with white light of 50 μmol of photons•m–2•s–1 and a continuous stream of air. Environmental Dark/High light/C02-Depletion