| PMID:24935866 |
Kopf M, Klähn S, Scholz I, Matthiessen JK, Hess WR, Voß B |
Comparative analysis of the primary transcriptome of Synechocystis sp. PCC 6803. |
DNA Res. 2014 Oct;21(5):527-39. |
The Synechocystis 6803 strain ‘PCC-M’24 was used. Liquid cultures were grown in 100 ml Erlenmeyer flasks using 75 ml of BG11 medium25 at 30°C under continuous white light illumination of 50–80 µmol quanta m−2 s−1 and a continuous stream of air to the desired growth phase (OD750 = 0.6–0.8). |
For the transcriptome analyses, cells were harvested from exponentially growing cultures which were then transferred to nine different conditions: (i) cold stress, 15°C for 30 min; (ii) heat stress, 42°C for 30 min; (iii) Ci depletion, cells were washed three times with carbon-free BG11 and cultivated further for 20 h; (iv) dark, no light for 12 h; (v) Fe2+ limitation, addition of iron-specific chelator desferrioxamine B (DFB) and further cultivation for 24 h; (vi) high light, 470 µmol quanta m−2 s−1 for 30 min; (vii) N depletion, cells were washed three times with nitrogen-free BG11 and cultivated further for 12 h; (viii) P depletion, cells were washed three times with phosphate-free BG11 and further incubated for 12 h; (ix) stationary phase, cells were grown until an OD750 of 4.7 was reached. |
Environmental |
Various stress conditions |