| PMID: 14612435 |
Wang HL, Postier BL, Burnap RL. |
Alterations in global patterns of gene expression in Synechocystis sp. PCC 6803 in response to inorganic carbon limitation and the inactivation of ndhR, a LysR family regulator. |
J Biol Chem. 2004 Feb 13;279(7):5739-51 |
For Ci-limited culture, Na2CO3 was omitted from BG11 medium. The modified BG11 was also buffered with 20 mM TES-KOH at pH 7.0, to inhibit the formation of HCO3-. The pre-cultures of the wild-type and mutant cells grown at logarithmic phase under CO2-enriched conditions (3% in air, v/v) were used to inoculate the experimental cultures at starting cell densities with an OD750 nm of 0.2. A relatively mild Ci stress was applied by switching the aeration from 3% CO2 in air (v/v) to air alone (350 ppm CO2, Ci-limited). |
The wild-type and mutant cells of Synechocystis 6803 were grown photoautotrophically at 32 °C (water bath) in BG11 medium buffered with 20 mM TES-KOH at pH 8.0, and bubbled with 3% CO2 in air (v/v). Continuous illumination was provided by fluorescent lamps with an incident flux of ∼50 μmol m-2 s-1 . Experimental cultures were grown in 600-ml batches in 1-liter Roux bottles (Corning) |
Environmental |
Nutrient limitation |