Publication or Contributor Authors Title Reference Treatment conditions Control/normal conditions Main source of perturbation Environmental factor tested
PMID: 15471853 Paithoonrangsarid K, Shoumskaya MA, Kanesaki Y, Satoh S, Tabata S, Los DA, Zinchenko VV, Hayashi H, Tanticharoen M, Suzuki I, Murata N. Five histidine kinases perceive osmotic stress and regulate distinct sets of genes in Synechocystis. J Biol Chem. 2004 Dec 17;279(51):53078-86 For exposure of cells to hyperosmotic stress, cells in a 50-ml culture were grown under standard conditions for 16 h (when the optical density at 730 nm of the culture was 0.3), and then 5.0 m sorbitol was added to the culture to give a final concentration at 0.5 m. The duration of incubation under osmotic stress was 20 min. Synechocystis PCC 6803 GT cells were grown photoautotrophically at 34 °C in BG-11 medium buffered with 20 mm HEPES-NaOH (pH 7.5) under continuous illumination from incandescent lamps at 70 μmol of photons/m2/s, with aeration by air that contained 1% CO2. mutant/Environmetal Oxidative stress