| PMID: 15743968 |
Kucho K, Okamoto K, Tsuchiya Y, Nomura S, Nango M, Kanehisa M, Ishiura M |
Global analysis of circadian expression in the cyanobacterium Synechocystis sp. strain PCC 6803 |
J Bacteriol. 2005 Mar;187(6):2190-9. |
To entrain the circadian clock, the culture were placed in darkness for 12 h and then kept in constant light conditions. Aliquots were taken every 4 h for 2 days (12 time points) |
Cells of Synechocystis sp. strain PCC 6803 were cultured in BG11 medium at 30°C under 91 μmol of white light illumination m−2 s−1 with bubbling of air and stirring. The optical density of the culture at 730 nm was maintained at approximately 0.35 by dilution with fresh BG-11 medium |
Environmental |
Circadian |